Data on flow-cytometric analysis of proliferation marker in cells during the period of culture. PCNA analysis of the cells was done after staining the cells with FITC-conjugated antibodies. (A) Representative histogram of unstained control. (B through E) histograms of PCNA-stained cells on day 1, day 4, day 8, and day 12, respectively, and (F) graphic representation of compiled data. Percentage of PCNA-positive cells from three replicate experiments is compiled, and the average ± SD of data from experiments carried out by using different donors’ PBMNCs is presented as a bar graph to compare percentage of PCNA-positive cells between the days of analysis. Days of analysis are marked on the x-axis, and percentage of positive cells on the y-axis of the bar diagram.