Figure 4From: Oxidative stress-induced Notch1 signaling promotes cardiogenic gene expression in mesenchymal stem cellsOxidative stress differentially modulates smooth muscle and fibroblast gene expression in mesenchymal stem cells (MSCs). MSCs were treated with either H2O2 for 1 week (0 to 100 μM; A through C) or GOX for 48 hours (0 to 5 mU/ml; D through F). Expression of the smooth muscle markers sm α-actin (A,D), sm22α (B,E), and the fibroblast marker vimentin (C, F) was determined with qRT-PCR. Values are expressed as mean ± SEM after normalizing gene expression to GAPDH expression. For A through C, n ≥ 3; and D-F, n = 6. *P < 0.05 and **P < 0.01 when compared with control (0 μM H2O2 or 0 mU/ml GOX) by one-way ANOVA followed by the Dunnett posttest.Back to article page