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Figure 4 | Stem Cell Research & Therapy

Figure 4

From: Oxidative stress-induced Notch1 signaling promotes cardiogenic gene expression in mesenchymal stem cells

Figure 4

Oxidative stress differentially modulates smooth muscle and fibroblast gene expression in mesenchymal stem cells (MSCs). MSCs were treated with either H2O2 for 1 week (0 to 100 μM; A through C) or GOX for 48 hours (0 to 5 mU/ml; D through F). Expression of the smooth muscle markers sm α-actin (A,D), sm22α (B,E), and the fibroblast marker vimentin (C, F) was determined with qRT-PCR. Values are expressed as mean ± SEM after normalizing gene expression to GAPDH expression. For A through C, n ≥ 3; and D-F, n = 6. *P < 0.05 and **P < 0.01 when compared with control (0 μM H2O2 or 0 mU/ml GOX) by one-way ANOVA followed by the Dunnett posttest.

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