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Figure 5 | Stem Cell Research & Therapy

Figure 5

From: Oxidative stress-induced Notch1 signaling promotes cardiogenic gene expression in mesenchymal stem cells

Figure 5

Oxidative stress increases expression of Notch1 and its downstream targets. Mesenchymal stem cells (MSCs) were treated with either H2O2 for 1 week (0 to 100 μM; A, B, D, E, F) or GOX for 48 hours (0 to 5 mU/ml; C,G through I). The mRNA expression levels of Notch1 (A, C), Hes5 (D, G), Hey1 (E, H), and the ligand Jagged1 (F, I) was determined with qRT-PCR. (B) Quantification of expression of activated Notch1 (NICD) with representative Western blot image. Values are expressed as mean ± SEM after normalizing gene expression and Western blots to GAPDH (A,B,D,E,F) or 18S (C,G, H,I) expression and protein, respectively. For A, B, and D through F, n ≥ 4; C and G through I, n ≥ 6; *P < 0.05 and **P < 0.01 when compared with control (0 μM H2O2 or 0 mU/ml GOX) by one-way ANOVA followed by the Dunnett posttest and Student unpaired t test for B .

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