Figure 7
H 2 O 2 and glucose oxidase (GOX) regulate cardiogenic gene expression in mesenchymal stem cells (MSCs) in both Notch1-dependent and -independent manners. MSCs were treated with 100 μM H2O2 ± Notch1 inhibitor DAPT (A through D) or with 5 mU/ml GOX ± siNotch1 (E through H). Expression of the cardiac marker αMHC (A, E), endothelial marker Flt1 (B,F), and the smooth muscle markers sm α-actin (C, G) and sm22α (D, H) were determined with qRT-PCR. Values are expressed as mean ± SEM after normalizing gene expression to GAPDH (A-D) or 18S (E-H) expression. n ≥ 4, *P < 0.05 and **P < 0.01 compared with control (0 μM H2O2), or #P < 0.05 compared with 100 μM H2O2 by one-way ANOVA followed by Bonferroni posttest.