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Figure 2 | Stem Cell Research & Therapy

Figure 2

From: Profiling of transcriptional and epigenetic changes during directed endothelial differentiation of human embryonic stem cells identifies FOXA2 as a marker of early mesoderm commitment

Figure 2

Validation of human embryonic stem cell–endothelial cell day 2 transcription factor differential expression. (A) Differential mRNA expression of 12 of the 22 transcription factors was confirmed by TaqMan® quantitative RT-PCR. (B) Differential expression of FOXA2 mRNA was confirmed in H1s and RC10s by TaqMan® quantitative RT-PCR. *P <0.05, **P <0.01 and ***P <0.001 versus pluripotent control. RQ, relative quantitation. (C) Immunocytofluorescent staining showing upregulation of FOXA2 protein expression at human embryonic stem cell–endothelial cell (hESC–SC) day 2. DAPI, 4′,6-diamidino-2-phenylindole; HSVEC, primary human saphenous vein endothelial cell. Scale bars = 20 μm.

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Stem Cell Research & Therapy

ISSN: 1757-6512