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Figure 1 | Stem Cell Research & Therapy

Figure 1

From: Murine amniotic fluid stem cells contribute mesenchymal but not epithelial components to reconstituted mammary ducts

Figure 1

Isolation, characterization, and differentiation potential of fetal stem cells derived from murine amniotic fluid. (a) Amniotic fluid stem cells (AFSs) were isolated at E13.5 and obtained by using a two-step culture model [9] from C57/B6-TG (ACTB-EGFP) and Rosa26 mice. (b) The freshly isolated and cultured AFSs exhibit pluripotency markers and exhibit no spontaneous differentiation in culture. (c) Histogram analysis of cell-surface markers showed that AFSs express embryonic and mesenchymal stem cell markers but lack the expression of hematopoietic cell markers. Grey line, isotype control. (d) Cultured AFSs exhibit an epithelial phenotype, as demonstrated by the co-expression of keratins and vimentin. (e) Differentiation of AFSs into mesodermal cell types after specific stimulation for 14 days is marked by the appearance of lipid granules (adipogenic) or mineralized matrix (osteogenic). The epithelial-stimulation protocol further potentiates the epithelial nature of AFSs (epithelial). Insets show higher magnification of the treated cells. Scale bars, 50 μm.

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