Figure 1

Changes in the DG expressions of genes controlling adult HPC neurogenesis during diabetes progression. On the day 5 of streptozotocin (STZ)-induced hyperglycemia, the brains were removed quickly, and the dentate gyrus (DG) region was microdissected to extract total RNA. For qPCR analysis, RNAs from the DG of control (n = 6) and diabetic rats (n = 6) were extracted and were subjected to qPCR analysis of gene expression of Sox2 and Nestin (stem cell marker genes), NeuroD1 (insulin-activating gene), insulin (proinsulin1), β-tubulin III (TUBB3, immature neuron marker gene), SYN1 (mature neuron marker gene), GFAP, GLIT1, SC1, and BYSL (astrocyte-marker genes). RNA samples were also prepared from rats 2 weeks and 2 months after the STZ induction.