Upregulated EAAT3 interacts with insulin under diabetes in HPC. (A) IHC of HPC neurons in molecular layer at DG of WT (wild-type) and DB rats. IHC images using antibodies of EAAT3 (red) and Insulin (green) are shown. (B) Magnified image at dotted-line area in Figure 4A is shown in separate panels. (C) Immunoprecipitation (IP) analysis of EAAT3 and insulin. The association between EAAT3 and insulin was specifically observed in the HPC from diabetic rats when EAAT3 expression was highly upregulated.