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Figure 4 | Stem Cell Research & Therapy

Figure 4

From: Endothelial microvascular networks affect gene-expression profiles and osteogenic potential of tissue-engineered constructs

Figure 4

Osteogenic potential of tissue-engineered constructs after 3 weeks of subcutaneous implantation. (A) Positive staining for alkaline phosphatase (purple) could be observed for both cell/scaffold constructs, as shown at 10× and 60× magnification. Scale bar: upper = 100 μm, lower = 20 μm. Calcified nodules were observed as positive Alizarin Red staining (red) shown at 4× and 40× magnification. Scale bar: upper = 100 μm, lower = 20 μm. (B) Gene-expression levels of osteogenic biomarkers alkaline phosphatase (ALP), osteopontin (OP), and collagen I (COL I) were evaluated with real-time RT-PCR, and increased expression of ALP was detected for MSC/EC constructs compared with empty controls. **P < 0.01; n = 6. (C) Quantification of Alizarin Red staining did not reveal significant differences between MSC and MSC/EC constructs. No positive staining was observed in the control scaffolds without cells; n = 4.

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