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Figure 5 | Stem Cell Research & Therapy

Figure 5

From: Endothelial microvascular networks affect gene-expression profiles and osteogenic potential of tissue-engineered constructs

Figure 5

Survival of implanted cells and the effect of local tissue signaling on osteogenesis. (A) Expression of human ki67 was upregulated at the moment of implantation after 1 week of culture in vitro, as well as at 1 week in vivo. At 3 weeks in vivo, the difference was no longer significant; n = 6. (B) Upper: Representative light micrographs (HE staining) of scaffolds penetrated with normal vascular loose connective tissue at 3 weeks subcutaneous implantation (20×). Lower: Masson trichrome staining (20×) could not detect osteoid formation in MSC or MSC/EC constructs at 3 weeks in vivo. (C) Upper: Representative fluorescent micrograph from the coculture group of cells positive for human vimentin at 3 weeks in vivo (20×). Lower: Positive control of MSC/EC implanted in a rat calvarial bone defect showing osteoid formation at 8 weeks (20×). Scale bars = 100 μm.

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