Figure 2

Immunofluorescent staining for inflammatory cells and enzymatic analysis of superoxide dismutase activity. Immunofluorescent (IF) staining for inflammatory cells and enzymatic analysis of superoxide dismutase (SOD) activity at 72 hours after the ischemia–reperfusion (IR) procedure (n = 8). (A) to (E) High-power field (HPF) IF microscopic findings (200×) showing the number of CD68⁺ cell infiltration (white arrows) in the five groups. Scale bars in right lower corner = 50 μm. (F) *P <0.0001 vs. other groups with different symbols; † vs. ‡ vs. ¶ vs. §, all P <0.05 for inter-group comparisons. (G) Analytical result of SOD activity in renal parenchyma at 72 hours after the IR procedure. *P <0.0001 vs. other groups with different symbols; † vs. ‡ vs. ¶ vs. §, all P <0.05 for inter-group comparisons. All statistical analyses were with one-way analysis of variance followed by Bonferroni multiple comparison post-hoc test. Different symbols among different groups indicate significance (at 0.05 level). ADMSC, adipose-derived mesenchymal stem cell; CsA, cyclosporine A.