Figure 3

Immunohistochemical staining for renal expressions of antioxidative markers at 72 hours after ischemia–reperfusion procedure. (A) to (E) Microscopic findings of immunohistochemical (IHC) stain (n =8, 200×) for glutathione reductase (GR)-positive cells (brown) in renal parenchyma of five groups. Scale bars in right lower corner = 50 μm. (F) *P <0.001 vs. other groups with different symbols; † vs. ‡ vs. ¶, all P <0.05 for inter-group comparisons. (G) to (K) Microscopic findings of IHC stain (200×) for glutathione peroxidase (GPx)-positive cells (brown) renal parenchyma of five groups. Scale bars in right lower corner = 50 μm. (L) *P <0.001 vs. other groups with different symbols; † vs. ‡ vs. ¶, all P <0.05 for inter-group comparisons. All statistical analyses performed using one-way analysis of variance followed by Bonferroni multiple comparison post-hoc test. Different symbols (*, †, ‡, ¶) among different groups indicate significance (at 0.05 level). ADMSC, adipose-derived mesenchymal stem cell; CsA, cyclosporine A.