Figure 8

Protein expression of inflammatory, reactive oxygen species, apoptotic and cytochrome C biomarkers in kidney. Changes in protein expression of inflammatory, reactive oxygen species (ROS), apoptotic and cytochrome C biomarkers in kidney at 72 hours after ischemia–reperfusion (IR) (n = 8). Protein expressions of TNFα (A), NF-κB (B), intercellular adhesion molecule (ICAM)-1 (C) and platelet-derived growth factor (PDGF) (D) were highest in the IR group and lowest in the sham control group, significantly higher in IR + cyclosporine A (CsA) and adipose-derived mesenchymal stem cell (ADMSC) groups than in the IR + CsA–ADMSC group. *P <0.001 vs. other groups with different symbols; * vs. † vs. ‡ vs. §, all P <0.05 for inter-group comparisons. Protein expressions of NADPH oxidase (NOX)-1 (E) and NOX-2 (F) were highest in the IR group and lowest in the sham control group, significantly higher in IR + CsA and ADMSC groups than in the IR + CsA–ADMSC group. *P <0.001 vs. other groups with different symbols; † vs. ‡ vs. § vs. ¶, all P <0.05 for inter-group comparisons. Protein expressions of mitochondrial Bax (G), cleaved caspase 3 (H) and cleaved poly(ADP-ribose) polymerase (PARP) (I) were highest in the IR group and lowest in the sham control group, significantly higher in IR + CsA and ADMSC groups than in IR + CsA–ADMSC group. For Bax and PARP: *P <0.001 vs. other groups with different symbols; † vs. ‡ vs. §, all P <0.05 for inter-group comparisons. The protein expression of cytosolic cytochrome C (J) was notably higher whereas mitochondrial cytochrome C (K) was markedly lower in the IR group than in other groups. *P <0.001 vs. other groups with different symbols; † vs. ‡ vs. §, all P <0.05 for inter-group comparisons. All statistical analyses performed using one-way analysis of variance followed by Bonferroni multiple comparison post-hoc test.