Figure 9

Western blot results of oxidative stress, antioxidant and endothelial function biomarkers in the kidney. Western blot results of oxidative stress, antioxidant and endothelial function biomarkers in the kidney at 72 hours after ischemia–reperfusion (IR) (n = 8). (A) Oxidative index (protein carbonyls) among five groups of animals (a = control, b = IR only, c = IR + CsA, d = IR + ADMSC, e = IR + CsA–ADMSC). (B) *P <0.0001 vs. other groups with different symbols; † vs. ‡ vs. §, all P <0.05 for inter-group comparisons. (Note: Right lane and left lane shown on the upper panel represent control oxidized molecular protein standard and protein molecular weight marker, respectively.) DNP, 1,3-dinitrophenylhydrazone. (C) Protein expressions of heme oxygenase (HO)-1 and (D) NAD(P)H quinone oxidoreductase (NQO)-1 among the five groups; *P <0.001 vs. other groups with different symbols; † vs. ‡ vs. § vs. ¶, all P <0.05 for inter-group comparisons. (E) Protein expression of endothelial nitric oxide synthase (eNOS). *P <0.001 vs. other groups with different symbols; † vs. ‡ vs. §, all P <0.05 for inter-group comparisons. Different symbols (*, †, ‡, ¶, §) among different indicate significance at the 0.05 level. ADMSC, adipose-derived mesenchymal stem cells; CsA, cyclosporine A.