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Figure 10 | Stem Cell Research & Therapy

Figure 10

From: Human Wharton’s jelly-derived mesenchymal stromal cells reduce renal fibrosis through induction of native and foreign hepatocyte growth factor synthesis in injured tubular epithelial cells

Figure 10

Human hepatocyte growth factor (HGF) mRNA and protein present in injured rat TECs after exposure to CM. (A) Representative micrographs indicating human HGF expression in the damaged rat TECs. Human HGF was present in cytoplasm of most of the WJ-MSCs and was used as a positive control. Rat TECs without exposure to CM, as a negative control, did not contain this heterogeneous protein. By contrast, it magically appeared in TECs exposed to CM for 24 hours or 48 hours. (a) WJ-MSCs; (b) TECs without exposure to CM; (c) TECs after 24 hours of exposure to CM; (d) TECs after 48 hours of exposure to CM; (B) Human HGF mRNA entering the injured rat TECs. Human HGF mRNA was detected in rat TECs exposed to CM for 3 hours or longer. Human HGF expressions in WJ-MSCs and rat TECs without exposure to CM were used as positive and negative controls, respectively. The Ct (threshold cycle) for human HGF gene and β-actin gene (rat or human) was determined for each cell sample. The quantification of the target gene was normalized by β-actin. Data are expressed as mean ± SD of three experiments.

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Stem Cell Research & Therapy

ISSN: 1757-6512