Figure 7

Densitometric analysis and gel photograph of HGF or TGF-β1 protein expression in kidney tissues at 1 day or 1 week after treatment. At 1 day, exposure to IRI stimulated the upregulation of HGF/TGF-β1 expression. However, by 1 week, the HGF/TGF-β1 expression came back to its normal status. By contrast, cell treatment or nephrectomy significantly upregulated the HGF/TGF-β1 expression through induction of HGF expression in damaged kidney, in favor of immediate injury repair. The density of each band was determined. Values in the graph are expressed as densitometric ratios of HGF/β-actin, TGF-β1/β-actin, or HGF/TGF-β1 as folds over control (sham-operated samples; dotted line). Data are shown as mean ± SD of three kidney samples for each experimental condition. *P < 0.05, IRI+WJ-MSCs versus IRI+VEHICLE; #P < 0.001, IRI+NEPHRECTOMY versus IRI+VEHICLE; xP < 0.05, IRI+VEHICLE versus SHAM. (a) Relative abundance of HGF/β-actin; (b) relative abundance of TGF-β1/β-actin; (c) relative abundance of HGF/TGF-β1; (d) gel photograph of HGF, TGF-β1, and β-actin protein expression.