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Table 2 Red blood cell and while blood cell quantification in whole blood and PRP1 fractions

From: Platelet-rich plasma preparation for regenerative medicine: optimization and quantification of cytokines and growth factors

Condition Volume (ml) RBC (106/μl) WBC (103/μl)
Whole blood 4.1 ± 1.4 4.05 ± 0.28 (100%) 4.38 ± 0.47 (100%)
1 (240 × g, 8 minutes, 16°C) 1.7 ± 0.2 0.02 ± 0.00 (0.19%) 0.02 ± 0.02 (0.21%)
2 (360 × g, 16 minutes, 16°C) 2.2 ± 0.1 0.00 ± 0.00 (0%) 0.00 ± 0.00 (0.12%)
3 (300 × g, 5 minutes, 12°C) 1.5 ± 0.4 0.03 ± 0.01 (0.24%) 0.03 ± 0.01 (0.28%)
4 (300 × g, 12 minutes, 5°C) 2.0 ± 0.1 0.01 ± 0.00 (0.12%) 0.01 ± 0.00 (0.12%)
5 (300 × g, 12 minutes, 12°C) 2.1 ± 0.2 0.01 ± 0.00 (0.13%) 0.01 ± 0.01 (0.08%)
  1. Values expressed as mean ± standard deviation (remaining amount of contaminant cells in PRP1, related to the initial amount and corrected by the corresponding volume). RBC red blood cell, WBC white blood cell, PRP1 platelet-rich plasma after the first centrifugation step. n = 3.