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Table 2 Red blood cell and while blood cell quantification in whole blood and PRP1 fractions

From: Platelet-rich plasma preparation for regenerative medicine: optimization and quantification of cytokines and growth factors

Condition

Volume (ml)

RBC (106/μl)

WBC (103/μl)

Whole blood

4.1 ± 1.4

4.05 ± 0.28 (100%)

4.38 ± 0.47 (100%)

1 (240 × g, 8 minutes, 16°C)

1.7 ± 0.2

0.02 ± 0.00 (0.19%)

0.02 ± 0.02 (0.21%)

2 (360 × g, 16 minutes, 16°C)

2.2 ± 0.1

0.00 ± 0.00 (0%)

0.00 ± 0.00 (0.12%)

3 (300 × g, 5 minutes, 12°C)

1.5 ± 0.4

0.03 ± 0.01 (0.24%)

0.03 ± 0.01 (0.28%)

4 (300 × g, 12 minutes, 5°C)

2.0 ± 0.1

0.01 ± 0.00 (0.12%)

0.01 ± 0.00 (0.12%)

5 (300 × g, 12 minutes, 12°C)

2.1 ± 0.2

0.01 ± 0.00 (0.13%)

0.01 ± 0.01 (0.08%)

  1. Values expressed as mean ± standard deviation (remaining amount of contaminant cells in PRP1, related to the initial amount and corrected by the corresponding volume). RBC red blood cell, WBC white blood cell, PRP1 platelet-rich plasma after the first centrifugation step. n = 3.