|
Cytokine
|
PRP2-Ca
|
PRP2-Thr
|
Result
|
|---|
|
PDGF-AA
|
6.674
|
5.931
|
Platelet-secreted factor
|
|
PDGF-AB
|
9.352
|
9.556
|
Platelet-secreted factor
|
|
PDGF-BB
|
8.072
|
7.886
|
Platelet-secreted factor
|
|
IGF-1
|
0.038
|
0.302
|
Plasmatic factor
|
|
TGF-β1
|
5.912
|
4.143
|
Platelet-secreted factor
|
|
TGF-β2
|
5.574
|
3.850
|
Platelet-secreted factor
|
|
TGF-β3
| | |
Not detected
|
|
EGF
|
9.243
|
8.386
|
Platelet-secreted factor
|
|
IL-5
|
2.568
|
2.539
|
Plasmatic factor
|
|
IL-6
|
0.303
|
0.388
|
Plasmatic factor
|
|
Eotaxin
|
5.829
|
6.169
|
a
|
|
bFGF
|
0.101
|
1.173
|
Plasmatic factor
|
|
G-CSF
|
0.081
|
0.097
|
Plasmatic factor
|
|
GM-CSF
|
0.711
|
0.716
|
Plasmatic factor
|
|
HGF
|
2.657
|
2.469
|
Plasmatic factor
|
|
IFNα
|
4.188
|
2.514
|
Platelet-secreted factor
|
|
IL-1β
|
0.480
|
0.590
|
Plasmatic factor
|
|
IL-2
|
0.869
|
0.882
|
Plasmatic factor
|
|
IL-2R
|
0.604
|
0.256
|
Plasmatic factor
|
|
IL-4
|
4.894
|
4.003
|
Platelet-secreted factor
|
|
IL-7
|
1.881
|
1.739
|
Plasmatic factor
|
|
IL-1RA
|
1.216
|
0.856
|
Plasmatic factor
|
|
IL-8
|
10.590
|
9.597
|
Platelet-secreted factor
|
|
IL-10
|
1.433
|
1.357
|
Plasmatic factor
|
|
IL-12
|
2.079
|
1.070
|
Plasmatic factor
|
|
IL-13
|
3.884
|
3.901
|
Platelet-secreted factor
|
|
IL-15
|
0.001
|
0.337
|
Plasmatic factor
|
|
IL-17
|
3.911
|
3.902
|
Platelet-secreted factor
|
|
VEGF
| | |
not detected
|
|
IFNγ
| | |
not detected
|
|
IP-10
|
3.131
|
3.288
|
Plasmatic factor
|
|
MCP-1
|
4.247
|
4.392
|
a
|
|
MIG
|
1.462
|
1.136
|
Plasmatic factor
|
|
MIP-1α
|
0.750
|
1.046
|
Plasmatic factor
|
|
MIP-1β
|
3.341
|
2.442
|
Plasmatic factor
|
|
RANTES
|
0.463
|
0.839
|
Plasmatic factor
|
|
TNFα
|
6.752
|
4.584
|
Platelet-secreted factor
|
- Determination of platelet-secreted cytokines using the Tukey post-hoc test. q-value = 3.68 (k = 3, n = 6, α = 0.05). bFGF basic fibroblast growth factor, EGF endothelial growth factor, GM-CSF granulocyte–macrophage colony-stimulating factor, HGF hepatocyte growth factor, IGF-1 insulin-like growth factor-1, IP-10 IFNγ-induced protein 10, MCP-1 monocyte chemoattractant protein-1, MIG IFNγ-induced monokine, MIP macrophage inflammatory protein, PDGF platelet-derived growth factor, PRP2 platelet-rich plasma after the second centrifugation step, PRP2-Ca calcium-activated PRP2, PRP2-Thr, calcium plus human thrombin-activated PRP2, TGF transforming growth factor, VEGF vascular endothelial growth factor. aEotaxin and MCP-1 showed statistically significant differences due to concentration reduction but they cannot be classified as platelet-secreted factors because their concentration was reduced, probably due to degradation by other products secreted following degranulation, or because they were associated with secreted molecules.
