Platelet-rich plasma preparation for regenerative medicine: optimization and quantification of cytokines and growth factors

Table 5 Statistical comparison (q-values) of cytokine concentration between plasma and activated PRP2

Cytokine	PRP2-Ca	PRP2-Thr	Result
PDGF-AA	6.674	5.931	Platelet-secreted factor
PDGF-AB	9.352	9.556	Platelet-secreted factor
PDGF-BB	8.072	7.886	Platelet-secreted factor
IGF-1	0.038	0.302	Plasmatic factor
TGF-β1	5.912	4.143	Platelet-secreted factor
TGF-β2	5.574	3.850	Platelet-secreted factor
TGF-β3			Not detected
EGF	9.243	8.386	Platelet-secreted factor
IL-5	2.568	2.539	Plasmatic factor
IL-6	0.303	0.388	Plasmatic factor
Eotaxin	5.829	6.169	^a
bFGF	0.101	1.173	Plasmatic factor
G-CSF	0.081	0.097	Plasmatic factor
GM-CSF	0.711	0.716	Plasmatic factor
HGF	2.657	2.469	Plasmatic factor
IFNα	4.188	2.514	Platelet-secreted factor
IL-1β	0.480	0.590	Plasmatic factor
IL-2	0.869	0.882	Plasmatic factor
IL-2R	0.604	0.256	Plasmatic factor
IL-4	4.894	4.003	Platelet-secreted factor
IL-7	1.881	1.739	Plasmatic factor
IL-1RA	1.216	0.856	Plasmatic factor
IL-8	10.590	9.597	Platelet-secreted factor
IL-10	1.433	1.357	Plasmatic factor
IL-12	2.079	1.070	Plasmatic factor
IL-13	3.884	3.901	Platelet-secreted factor
IL-15	0.001	0.337	Plasmatic factor
IL-17	3.911	3.902	Platelet-secreted factor
VEGF			not detected
IFNγ			not detected
IP-10	3.131	3.288	Plasmatic factor
MCP-1	4.247	4.392	^a
MIG	1.462	1.136	Plasmatic factor
MIP-1α	0.750	1.046	Plasmatic factor
MIP-1β	3.341	2.442	Plasmatic factor
RANTES	0.463	0.839	Plasmatic factor
TNFα	6.752	4.584	Platelet-secreted factor

Determination of platelet-secreted cytokines using the Tukey post-hoc test. q-value = 3.68 (k = 3, n = 6, α = 0.05). bFGF basic fibroblast growth factor, EGF endothelial growth factor, GM-CSF granulocyte–macrophage colony-stimulating factor, HGF hepatocyte growth factor, IGF-1 insulin-like growth factor-1, IP-10 IFNγ-induced protein 10, MCP-1 monocyte chemoattractant protein-1, MIG IFNγ-induced monokine, MIP macrophage inflammatory protein, PDGF platelet-derived growth factor, PRP2 platelet-rich plasma after the second centrifugation step, PRP2-Ca calcium-activated PRP2, PRP2-Thr, calcium plus human thrombin-activated PRP2, TGF transforming growth factor, VEGF vascular endothelial growth factor. ^aEotaxin and MCP-1 showed statistically significant differences due to concentration reduction but they cannot be classified as platelet-secreted factors because their concentration was reduced, probably due to degradation by other products secreted following degranulation, or because they were associated with secreted molecules.

ISSN: 1757-6512