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Table 5 Statistical comparison (q-values) of cytokine concentration between plasma and activated PRP2

From: Platelet-rich plasma preparation for regenerative medicine: optimization and quantification of cytokines and growth factors

Cytokine PRP2-Ca PRP2-Thr Result
PDGF-AA 6.674 5.931 Platelet-secreted factor
PDGF-AB 9.352 9.556 Platelet-secreted factor
PDGF-BB 8.072 7.886 Platelet-secreted factor
IGF-1 0.038 0.302 Plasmatic factor
TGF-β1 5.912 4.143 Platelet-secreted factor
TGF-β2 5.574 3.850 Platelet-secreted factor
TGF-β3    Not detected
EGF 9.243 8.386 Platelet-secreted factor
IL-5 2.568 2.539 Plasmatic factor
IL-6 0.303 0.388 Plasmatic factor
Eotaxin 5.829 6.169 a
bFGF 0.101 1.173 Plasmatic factor
G-CSF 0.081 0.097 Plasmatic factor
GM-CSF 0.711 0.716 Plasmatic factor
HGF 2.657 2.469 Plasmatic factor
IFNα 4.188 2.514 Platelet-secreted factor
IL-1β 0.480 0.590 Plasmatic factor
IL-2 0.869 0.882 Plasmatic factor
IL-2R 0.604 0.256 Plasmatic factor
IL-4 4.894 4.003 Platelet-secreted factor
IL-7 1.881 1.739 Plasmatic factor
IL-1RA 1.216 0.856 Plasmatic factor
IL-8 10.590 9.597 Platelet-secreted factor
IL-10 1.433 1.357 Plasmatic factor
IL-12 2.079 1.070 Plasmatic factor
IL-13 3.884 3.901 Platelet-secreted factor
IL-15 0.001 0.337 Plasmatic factor
IL-17 3.911 3.902 Platelet-secreted factor
VEGF    not detected
IFNγ    not detected
IP-10 3.131 3.288 Plasmatic factor
MCP-1 4.247 4.392 a
MIG 1.462 1.136 Plasmatic factor
MIP-1α 0.750 1.046 Plasmatic factor
MIP-1β 3.341 2.442 Plasmatic factor
RANTES 0.463 0.839 Plasmatic factor
TNFα 6.752 4.584 Platelet-secreted factor
  1. Determination of platelet-secreted cytokines using the Tukey post-hoc test. q-value = 3.68 (k = 3, n = 6, α = 0.05). bFGF basic fibroblast growth factor, EGF endothelial growth factor, GM-CSF granulocyte–macrophage colony-stimulating factor, HGF hepatocyte growth factor, IGF-1 insulin-like growth factor-1, IP-10 IFNγ-induced protein 10, MCP-1 monocyte chemoattractant protein-1, MIG IFNγ-induced monokine, MIP macrophage inflammatory protein, PDGF platelet-derived growth factor, PRP2 platelet-rich plasma after the second centrifugation step, PRP2-Ca calcium-activated PRP2, PRP2-Thr, calcium plus human thrombin-activated PRP2, TGF transforming growth factor, VEGF vascular endothelial growth factor. aEotaxin and MCP-1 showed statistically significant differences due to concentration reduction but they cannot be classified as platelet-secreted factors because their concentration was reduced, probably due to degradation by other products secreted following degranulation, or because they were associated with secreted molecules.