Figure 3From: Conditionally immortalized stem cell lines from human spinal cord retain regional identity and generate functional V2a interneurons and motorneuronsCharacterization of SPC-01 differentiation. Differentiation of SPC-01 by removal of growth factors and 4-OHT for 7 days gives rise to tau+ neurons (left panel). Consistent with the homeodomain transcription-factor profile observed in the undifferentiated cells, a subset of these tau+ neurons coexpress LHX3 and CHX10, indicative of V2a interneurons (middle panel). However, a subpopulation of NKX6.1+/CHX10- neurons can also be observed (right panel, white arrows).Back to article page