Figure 8

Expression of pro-angiogenic factors by human bone marrow-derived mesenchymal stem cells. (a) Immunofluorescence analysis of pro-angiogenic factor expression in human bone marrow-derived mesenchymal stem cells (hBM-MSCs) after co-culture with DU145 cells. DAPI (for nuclei staining) is blue; transforming growth factor (TGF)-β and vascular endothelial growth factor (VEGF) detected in green; IL-6 detected in red. Scale bar, 100 μm. (b) Real-time quantitative PCR for pro-angiogenic factor relative mRNA levels to GAPDH in hBM-MSCs, hBM-MSCs co-cultured with DU145 cells and hBM-MSCs cultured in conditioned medium of DU145 cells (n = 6 per group; *P <0.05). MIP-2, macrophage inflammatory protein-2.