Figure 3

Initial corneal epithelial differentiation of OFSCs was observed in the limbal epithelium. (A) Cytokeratin (CK) 19 was primarily stained in the limbal area, but neither human immunoglobulin G (hIgG) nor CK19 co-expressing cells were found in the limbal area after topical OFSC treatment of the limbal-damaged cornea for three days (B). CK3 expression was noted in both the central (C, E) and limbal (D, F) cornea. Three days after topical OFSC treatment, some hIgG and CK3 co-expressing cells were observed in the limbal epithelium (F), but not in the (D) central epithelium.