Figure 3

The expression of pluripotent potent and neural markers during the induction process of the iPSCs to NSCs. Immunocytochemistry staining shows that the spheres expressed the neural precursor markers Nestin (A), Sox2 (B) induced by RA and serum-free medium, and after culture, in serum medium; those spheres expressed the neuron marker β-tubulin (C) and astrocyte marker GFAP (D). The adherent cells expressed the neural precursor markers Nestin (E), Sox2 (F), and differentiated into neurons and astrocytes in the same conditions (G, H). The expression of pluripotent genes Sox2 and Oct4 during the induction process in the iPSCs and ESCs (I); the iPSC-derived NSCs expressed high levels of Nestin, β-tubulin, and GFAP during the induction process and showed a similar tendency to the ESC-derived NSCs (J, *compared with ESCs; P < 0.05).