Figure 2

Effect of culture medium on the mesenchymal differentiation potential of PLCSCs. PLCSCs were cultured in KM or MM and put through adipogenic, chondrogenic, and osteogenic differentiation protocols, and comparative qPCR analysis (a through i) and histologic staining (j through o) were performed. Gene expression and staining for adipogenesis (a, FASN, d, PLIN;, g, PPARG, j and m, Oil Red O staining); chondrogenesis (b, ACAN, e, COMP, h, SOX9, k and n, Alcian blue staining); osteogenesis (c, BMP4, f, BMP6, I, OPG, l and o, Alizarin red staining). Values represented as mean ± SD, experiment repeated in triplicate, each with n = 3. P values for gene expression are indicated, and statistically significant values are represented (*P < 0.01; **P < 0.001). Experiment was repeated in triplicate. Positive adipogenic, chondrogenic, and osteogenic staining are represented by the presence of lipids (red), glycosaminoglycans (blue), and extracellular calcium deposits (red), respectively. Magnification, 400×.