|
Cell-surface markers
|
Mean % of positive cells cultured in
|
Mean % of positive cells in subpopulation
|
|---|
| |
MM
|
KM
|
A
|
B
|
C
|
|---|
|
ISCT Ppsitive criteria (≥95%)
1
|
|
CD73
|
97.13 ± 0.81 ✓
|
82.47 ± 2.73 ✕
|
97.13 ± 0.81 ✓
|
64.00 ± 4.00 ✕
|
73.00 ± 3.00 ✕
|
|
CD90
|
98.13 ± 2.71 ✓
|
99.97 ± 0.06 ✓
|
99.47 ± 0.40 ✓
|
99.97 ± 0.06 ✓
|
97.67 ± 1.53 ✓
|
|
CD105
|
96.67 ± 1.53 ✓
|
21.33 ± 2.08 ✕
|
72.00 ± 6.00 ✕
|
50.00 ± 10.00 ✕
|
46.67 ± 20.82 ✕
|
|
ISCT negative criteria (<2%)
|
|
CD11b
|
- ✓
|
- ✓
|
- ✓
|
- ✓
|
- ✓
|
|
CD19
|
- ✓
|
0.54 ± 0.42 ✓
|
- ✓
|
0.54 ± 0.42 ✓
|
- ✓
|
|
CD34
|
- ✓
|
- ✓
|
- ✓
|
- ✓
|
- ✓
|
|
CD45
|
- ✓
|
1.80 ±0.26 ✓
|
4.00 ± 2.00 ✕
|
- ✓
|
- ✓
|
|
HLA DP,DQ,DR
|
- ✓
|
- ✓
|
- ✓
|
- ✓
|
- ✓
|
|
Additional Markers
|
|
ABCG2
|
2.05 ± 1.69
|
0.53 ± 0.04
|
10.00 ± 2.00
|
-
|
-
|
|
CD13
|
56.67 ± 28.87
|
43.00 ± 1.00
|
70.00 ± 17.32
|
2.20 ± 0.20
|
3.27 ± 0.25
|
|
CD29
|
97.93 ± 2.55
|
97.23 ± 2.66
|
97.93 ± 2.55
|
96.90 ± 2.15
|
96.23 ± 1.33
|
|
CD44
|
97.00 ± 1.00
|
90.33 ± 0.58
|
96.00 ± 2.00
|
99.33 ± 0.58
|
99.00 ± 1.00
|
|
CD49e
|
77.00 ± 1.00
|
50.00 ± 5.00
|
59.07 ± 12.18
|
48.33 ± 1.53
|
30.00 ± 10.00
|
|
CD49f
|
-
|
-
|
-
|
-
|
-
|
|
CD104
|
-
|
-
|
-
|
-
|
-
|
|
CD106
|
2.60 ± 0.26
|
0.6 ± 0.26
|
-
|
1.50 ± 0.46
|
1.30 ± 0.03
|
|
CD133
|
0.88 ± 0.15
|
0.59 ±0.09
|
0.88 ± 0.15
|
0.59 ± 0.09
|
0.66 ± 0.15
|
|
CD271
|
-
|
0.56 ±0.48
|
4.80 ± 1.85
|
-
|
-
|
|
HLA ABC
|
97.90 ± 1.14
|
84.00 ± 1.00
|
42.00 ± 3.00
|
7.00 ± 2.00
|
4.00 ± 1.00
|
|
Stro-1
|
0.56 ± 0.27
|
0.47 ± 0.41
|
0.56 ± 0.27
|
-
|
-
|
|
Vimentin
|
17.67 ± 2.52
|
8.00 ±1.00
|
13.33 ± 5.77
|
14.00 ± 2.00
|
10.00 ± 2.00
|
|
Control markers
|
|
CK 3/12
|
0.54 ± 0.23
|
-
|
0.60 ± 0.23
|
-
|
-
|
|
CK 14
|
0.63 ± 0.21
|
-
|
0.81 ± 0.21
|
-
|
-
|
|
CK 19
|
0.78 ± 0.29
|
-
|
0.72 ± 0.29
|
-
|
-
|
- PLCSCs cultured in MM and KM were analyzed for cell-surface markers at P3. Subpopulations (A: CD34+CD105+, B: CD34-CD105+, C: CD34-CD105-) were analyzed after cell sorting and culture in MM up to P3. ✓ denotes that the cells have fulfilled the ISCT criteria, ✓ denotes that the cells did not conform to the ISCT criteria. Values of ≤0.5% are denoted by dash (-). Values are represented by mean standard deviation (in brackets). Flow cytometry was performed on three separate donor-cell populations.
