Table 1 Cell-surface marker profiling of PLCSCs and isolated subpopulations for ISCT criteria and additional markers
Cell-surface markers | Mean % of positive cells cultured in | Mean % of positive cells in subpopulation | |||
|---|---|---|---|---|---|
MM | KM | A | B | C | |
ISCT Ppsitive criteria (≥95%) 1 | |||||
CD73 | 97.13 ± 0.81 ✓ | 82.47 ± 2.73 ✕ | 97.13 ± 0.81 ✓ | 64.00 ± 4.00 ✕ | 73.00 ± 3.00 ✕ |
CD90 | 98.13 ± 2.71 ✓ | 99.97 ± 0.06 ✓ | 99.47 ± 0.40 ✓ | 99.97 ± 0.06 ✓ | 97.67 ± 1.53 ✓ |
CD105 | 96.67 ± 1.53 ✓ | 21.33 ± 2.08 ✕ | 72.00 ± 6.00 ✕ | 50.00 ± 10.00 ✕ | 46.67 ± 20.82 ✕ |
ISCT negative criteria (<2%) | |||||
CD11b | - ✓ | - ✓ | - ✓ | - ✓ | - ✓ |
CD19 | - ✓ | 0.54 ± 0.42 ✓ | - ✓ | 0.54 ± 0.42 ✓ | - ✓ |
CD34 | - ✓ | - ✓ | - ✓ | - ✓ | - ✓ |
CD45 | - ✓ | 1.80 ±0.26 ✓ | 4.00 ± 2.00 ✕ | - ✓ | - ✓ |
HLA DP,DQ,DR | - ✓ | - ✓ | - ✓ | - ✓ | - ✓ |
Additional Markers | |||||
ABCG2 | 2.05 ± 1.69 | 0.53 ± 0.04 | 10.00 ± 2.00 | - | - |
CD13 | 56.67 ± 28.87 | 43.00 ± 1.00 | 70.00 ± 17.32 | 2.20 ± 0.20 | 3.27 ± 0.25 |
CD29 | 97.93 ± 2.55 | 97.23 ± 2.66 | 97.93 ± 2.55 | 96.90 ± 2.15 | 96.23 ± 1.33 |
CD44 | 97.00 ± 1.00 | 90.33 ± 0.58 | 96.00 ± 2.00 | 99.33 ± 0.58 | 99.00 ± 1.00 |
CD49e | 77.00 ± 1.00 | 50.00 ± 5.00 | 59.07 ± 12.18 | 48.33 ± 1.53 | 30.00 ± 10.00 |
CD49f | - | - | - | - | - |
CD104 | - | - | - | - | - |
CD106 | 2.60 ± 0.26 | 0.6 ± 0.26 | - | 1.50 ± 0.46 | 1.30 ± 0.03 |
CD133 | 0.88 ± 0.15 | 0.59 ±0.09 | 0.88 ± 0.15 | 0.59 ± 0.09 | 0.66 ± 0.15 |
CD271 | - | 0.56 ±0.48 | 4.80 ± 1.85 | - | - |
HLA ABC | 97.90 ± 1.14 | 84.00 ± 1.00 | 42.00 ± 3.00 | 7.00 ± 2.00 | 4.00 ± 1.00 |
Stro-1 | 0.56 ± 0.27 | 0.47 ± 0.41 | 0.56 ± 0.27 | - | - |
Vimentin | 17.67 ± 2.52 | 8.00 ±1.00 | 13.33 ± 5.77 | 14.00 ± 2.00 | 10.00 ± 2.00 |
Control markers | |||||
CK 3/12 | 0.54 ± 0.23 | - | 0.60 ± 0.23 | - | - |
CK 14 | 0.63 ± 0.21 | - | 0.81 ± 0.21 | - | - |
CK 19 | 0.78 ± 0.29 | - | 0.72 ± 0.29 | - | - |