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Table 1 Cell-surface marker profiling of PLCSCs and isolated subpopulations for ISCT criteria and additional markers

From: Characterization of corneal stromal stem cells with the potential for epithelial transdifferentiation

Cell-surface markers Mean % of positive cells cultured in Mean % of positive cells in subpopulation
  MM KM A B C
ISCT Ppsitive criteria (≥95%) 1
CD73 97.13 ± 0.81 82.47 ± 2.73 97.13 ± 0.81 64.00 ± 4.00 73.00 ± 3.00
CD90 98.13 ± 2.71 99.97 ± 0.06 99.47 ± 0.40 99.97 ± 0.06 97.67 ± 1.53
CD105 96.67 ± 1.53 21.33 ± 2.08 72.00 ± 6.00 50.00 ± 10.00 46.67 ± 20.82
ISCT negative criteria (<2%)
CD11b - - - - -
CD19 - 0.54 ± 0.42 - 0.54 ± 0.42 -
CD34 - - - - -
CD45 - 1.80 ±0.26 4.00 ± 2.00 - -
HLA DP,DQ,DR - - - - -
Additional Markers
ABCG2 2.05 ± 1.69 0.53 ± 0.04 10.00 ± 2.00 - -
CD13 56.67 ± 28.87 43.00 ± 1.00 70.00 ± 17.32 2.20 ± 0.20 3.27 ± 0.25
CD29 97.93 ± 2.55 97.23 ± 2.66 97.93 ± 2.55 96.90 ± 2.15 96.23 ± 1.33
CD44 97.00 ± 1.00 90.33 ± 0.58 96.00 ± 2.00 99.33 ± 0.58 99.00 ± 1.00
CD49e 77.00 ± 1.00 50.00 ± 5.00 59.07 ± 12.18 48.33 ± 1.53 30.00 ± 10.00
CD49f - - - - -
CD104 - - - - -
CD106 2.60 ± 0.26 0.6 ± 0.26 - 1.50 ± 0.46 1.30 ± 0.03
CD133 0.88 ± 0.15 0.59 ±0.09 0.88 ± 0.15 0.59 ± 0.09 0.66 ± 0.15
CD271 - 0.56 ±0.48 4.80 ± 1.85 - -
HLA ABC 97.90 ± 1.14 84.00 ± 1.00 42.00 ± 3.00 7.00 ± 2.00 4.00 ± 1.00
Stro-1 0.56 ± 0.27 0.47 ± 0.41 0.56 ± 0.27 - -
Vimentin 17.67 ± 2.52 8.00 ±1.00 13.33 ± 5.77 14.00 ± 2.00 10.00 ± 2.00
Control markers
CK 3/12 0.54 ± 0.23 - 0.60 ± 0.23 - -
CK 14 0.63 ± 0.21 - 0.81 ± 0.21 - -
CK 19 0.78 ± 0.29 - 0.72 ± 0.29 - -
  1. PLCSCs cultured in MM and KM were analyzed for cell-surface markers at P3. Subpopulations (A: CD34+CD105+, B: CD34-CD105+, C: CD34-CD105-) were analyzed after cell sorting and culture in MM up to P3. denotes that the cells have fulfilled the ISCT criteria, denotes that the cells did not conform to the ISCT criteria. Values of ≤0.5% are denoted by dash (-). Values are represented by mean standard deviation (in brackets). Flow cytometry was performed on three separate donor-cell populations.