Neuron-like cell differentiation from HUMSCs by D609 induction. (A) Representative images of HUMSCs and HUMSC-NCs obtained by an inverted phase-contrast microscopy. Scale bar, 50 μm. (B) Immunocytochemistry staining for neuronal markers NSE (red) and MAP2 (red) in HUMSCs and HUMSC-NCs. Cells were fixed and stained with rabbit anti-human NSE IgG (1:200) or rabbit anti-human MAP2 IgG (1:200). Fluorescent dye-conjugated secondary antibody, goat anti-rabbit IgG-TRITC, was used to visualize the cells. Nuclei were stained with DAPI (blue). The images were captured with a camera system connected to a fluorescence microscope (Olympus 1x71S1F-3, Japan). Scale bar, 50 μm.