Figure 4

The combination of hBM-MSCs and minocycline decreases demyelination in the EAE mouse spinal cord. (A) LFB- (upper panel) and MBP-stained (bottom panel) spinal cord sections were assessed to detect the extent of demyelination in EAE mice. The arrows indicate demyelinated areas. Scale bar, 200 μm. (B) Statistical analysis demonstrated that combination treatment significantly reduced demyelination compared with hBM-MSCs or minocycline treatment alone (P < 0.05). (C) Combination treatment also significantly preserved the number of MBP-positive cells compared with hBM-MSCs or minocycline treatment alone (P < 0.001). Columns, mean; bars, SEM. *P < 0.05, **P < 0.01, ***P < 0.001, one-way ANOVA with post hoc Bonferroni corrections. The results are representative of three independent experiments.