Figure 9

Immuncytochemical analysis of expressed proteins in mammary gland-derived cells. The staining for nestin (red), Ki67 (green), cytokeratin19 (CK19, red), neurofilaments (NF, green) and alpha-smooth muscle actin (αSMA) are exemplarily shown for a female mammary gland-derived cell population (MGDC 3, left panel) and a male mammary gland-derived cell population (MGDC2, right panel), each in passage 3 (left row of each panel) and passage 9 (right row of each panel). Nuclei are stained blue with 4′,6-diamidino-2-phenylindole. Bar = 100 μm. Magnification: 200 ×.