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Figure 2 | Stem Cell Research & Therapy

Figure 2

From: Efficient, high-throughput transfection of human embryonic stem cells

Figure 2

Transfected H9 express markers of pluripotency. (a) Fluorescence image of GFP transfected cells (green) that express Oct4 (red). (b) Fluorescence image of GFP transfected cells (green) that express SSEA4 (red). (c) FACS analysis for markers of pluripotency 24 hours after electroporation demonstrate that control and transfected cells expressed similar amounts of SSEA4 (blue bars; 74.4 ± 4.3% vs. 72.8 ± 4.4%; P > 0.5). In addition, of the GFP expressing cells 79.9 ± 4.7% also express SSEA4 (green bars).

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