Figure 5From: Efficient, high-throughput transfection of human embryonic stem cellsStable hESC cell line expressing red fluorescent protein. RNJ9 cells transfected with EF1α-RFP/PGK-Neo were selected with 50 μg/ml G418 for two weeks after transfection and then grown for 10 passages. (a) Fluorescence images of dapi (blue), SSEA4 (green; in fixed cells SSEA4 staining appears punctuate due to the disruption of the cell membrane by paraformaldehyde, and RFP (red). (b) Fluorescence images of dapi (blue), Oct4 (green), and RFP (red).Back to article page