Table 1 Efficiency of stable clone generation based on resistance markers and their promoters
From: Efficient, high-throughput transfection of human embryonic stem cells
Vector | Resistance marker | Transcriptional control | Selective drug (dose) | Frequency of stable integrants5 |
|---|---|---|---|---|
pPGK-Puro | Puro | PGK1 | Puromycin (0.5 μg/ml) | >8 × 10-5 |
pTracer-BSD | BSD (GFP fusion) | EF1α2 | Blasticidin-S (2.0 μg/ml) | 4.9 × 10-5 |
pCMV-BSD | BSD | CMV3 | Blasticidin-S (2.0 μg/ml) | 6.3 × 10-6 |
pBM14 | Neo | PGK1 | Geneticin/G418 (50 μg/ml) | 6.0 × 10-6 |
pEGFP-C3 | Neo | SV404 | Geneticin/G418 (50 μg/ml) | 6.3 × 10-6 |
pRNAT-U6.1 (GFP) | Hyg | SV404 | Hygromycin-B (10 to 100 μg/ml) | <1.0 × 10-6 (none recovered) |