Skip to main content

Table 1 Efficiency of stable clone generation based on resistance markers and their promoters

From: Efficient, high-throughput transfection of human embryonic stem cells

Vector Resistance marker Transcriptional control Selective drug (dose) Frequency of stable integrants5
pPGK-Puro Puro PGK1 Puromycin (0.5 μg/ml) >8 × 10-5
pTracer-BSD BSD (GFP fusion) EF1α2 Blasticidin-S (2.0 μg/ml) 4.9 × 10-5
pCMV-BSD BSD CMV3 Blasticidin-S (2.0 μg/ml) 6.3 × 10-6
pBM14 Neo PGK1 Geneticin/G418 (50 μg/ml) 6.0 × 10-6
pEGFP-C3 Neo SV404 Geneticin/G418 (50 μg/ml) 6.3 × 10-6
pRNAT-U6.1 (GFP) Hyg SV404 Hygromycin-B (10 to 100 μg/ml) <1.0 × 10-6 (none recovered)
  1. 1Murine phosphoglycerate kinase promoter/enhancer.
  2. 2Human elongation factor 1-alpha promoter/enhancer.
  3. 3Cytomegalovirus immediate-early promoter/enhancer.
  4. 4Simian virus-40 promoter/origin region.
  5. 5Values shown represent means of two to six experiments.