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Figure 3 | Stem Cell Research & Therapy

Figure 3

From: Adipogenesis of adipose-derived stem cells may be regulated via the cytoskeleton at physiological oxygen levels in vitro

Figure 3

Adipogenic gene expression as a function of oxygen levels and cytoskeletal inhibition. Adipose-derived stem cells (ASCs) were cultured under 20% O2 (white) and 5% O2 (black) conditions, and treated with cytochalasin D (cyto), blebbistatin (bleb) or vehicle control (control). All transcript levels were normalized to 18 S ribosomal RNA, and then to 20% O2, non-inhibited (control) samples on Day 7. Peroxisome proliferator-activated receptor γ (PPARγ) (A, B), lipoprotein lipase (LPL) (C, D) and fatty acid binding protein 4 (FABP4) (E, F) expression were analyzed on days 7 and 21. Culturing under 5% O2 conditions significantly decreased FABP4 in the non-inhibited control group compared to the 20% O2 control group at Day 21. Cytoskeletal inhibitors significantly increased adipogenic gene expression regardless of oxygen levels or time point as compared to non-inhibited controls. Cytoskeletal inhibitors applied under 5% O2 conditions led to a significant increase in PPARγ (A) and FABP4 (E) gene expression as compared to the corresponding 20% O2 inhibited samples at Day 7. Values are reported as mean ± SEM of n = 3 donors; * = significant change relative to non-inhibited control under the same oxygen condition with P <0.05; † = significant change relative to corresponding 20% O2 sample with P <0.05; • = significant change relative to corresponding Day 7 sample with P <0.05. Significance was only reported for trends that were consistent for all three cell donors.

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