Figure 5
Glycerol-3-phosphate dehydrogenase (GPDH) activity as a function of oxygen levels and cytoskeletal inhibition. GPDH activity was analyzed after 14 and 21 days of differentiation. Adipose-derived stem cells (ASCs) were cultured under 20% O2 (white) and 5% O2 (black) conditions, and treated with cytochalasin D (cyto), blebbistatin (bleb) or vehicle control (control). In control groups, 5% O2, reduced GPDH activity after 14 (A) and significantly at 21 (B) days, compared to 20% O2. Conversely, cytochalasin D and blebbistatin increased GPDH activity compared to non-inhibited samples, regardless of oxygen condition after 14 (A) and 21 (B) days. Values were reported as mean ± SEM with n = 3 cell donors; * = significant change relative to non-inhibited control under the same oxygen condition with P <0.05; †= significant change relative to corresponding 20% O2 sample with P <0.05. Significance was only reported for trends that were consistent for all three cell donors.