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Figure 5 | Stem Cell Research & Therapy

Figure 5

From: Isolation method and xeno-free culture conditions influence multipotent differentiation capacity of human Wharton’s jelly-derived mesenchymal stem cells

Figure 5

Flow cytometry analysis of WJ explant- and pellet-derived MSCs cultured in XF medium. MSC markers expression by WJ explant (1)- and pellet (2)-derived MSCs at P2; (3) Comparative MFI expression of CD44 and/or CD73 markers in: (3a) Explant-derived MSCs at P2 (E-P2) versus P5 (E-P5); (3b) Pellet-derived MSCs at P2 (P-P2) versus P5 (P-P5), as compared to freshly isolated pellet-derived WJ cells (P-P0) and a commercially available NUFF1 human fibroblast cell line (FIB); (3c) explant-derived MSCs at P2 (E-P2) versus pellet-derived MSCs at P2 (P-P2), as compared to freshly isolated pellet-derived WJ cells (P-P0). MFI, median fluorescence intensity; MSCs, mesenchymal stem cells; P, passage; WJ, Wharton’s jelly; XF, xeno-free.

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Stem Cell Research & Therapy

ISSN: 1757-6512