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Figure 7 | Stem Cell Research & Therapy

Figure 7

From: Isolation method and xeno-free culture conditions influence multipotent differentiation capacity of human Wharton’s jelly-derived mesenchymal stem cells

Figure 7

Morphology (I) and tube formation capacity (II) of WJ-derived cells used into endothelial differentiation assays. A – Group A cells isolated and expanded until P2 in the XF medium, and then subcultured for five consecutive passages in endothelial medium; B – Group B cells isolated and subcultured for five consecutive passages in endothelial medium. Explant- and pellet-derived cells belonging to group A did not show morphological (I.A – 1a/1b and 3a/3b, respectively) and functional (II.A – 1 and 4, respectively) features of endothelial cells. Within group B, explant-derived cells also neither exhibited morphological (I.B – 2a/2b), nor functional (II.B – 2) characteristics of endothelial cells, whereas pellet-derived cells presented endothelial characteristics both at morphological (I.B – 4a/4b) and functional (II.B – 5a/5b) levels; II.B – 3, positive control for Matrigel tube formation assay, represented by a commercially available HUVEC line. Higher magnifications of corresponding pictures are pointed by arrows. HUVEC, human umbilical vein endothelial cell; MSCs, mesenchymal stem cells; P, passage; WJ, Wharton’s jelly; XF, xeno-free.

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