Figure 1

Schematic view of EPI-NCSC isolation and culture. The whisker pad (B) of 3-week-old mouse (A) was cut, and hair follicles (C) were dissected. The bulge region (D) was rolled out from the capsule of hair follicle and explanted in α-MEM, 10% FBS, and 5% CEE (primary explant). After 10 days, migrated EPI-NCSCs were trypsinized and cultured in α-MEM supplemented with 10% CEE and FGF (expansion medium) or cells cultivated in only CSF for 72 hours.