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Figure 4 | Stem Cell Research & Therapy

Figure 4

From: The influence of cerebrospinal fluid on epidermal neural crest stem cells may pave the path for cell-based therapy

Figure 4

Expression of pertinent genes of EPI-NCSCs. (A) Immunocytochemistry of Nestin, β-tubulin ІІІ, and GFAP (all green) in expanded EPI-NCSCs after 72 hours of cultivation in CSF and expansion medium. All nuclei are stained red with PI. (B) Expression of Nestin, β-tubulin ІІІ, and GFAP genes in EPI-NCSCs was compared with primary explant by qRT-PCR, and the ΔΔ Ct method was used to determine alterations in expression levels after cultivation in expansion medium and CSF. qRT-PCR showed a decreased trend in expression of all three investigated genes after 72 hours of cultivation in CSF. *P < 0.05; ***P < 0.001. Error bars, 95% Cl.

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