Figure 1From: A comparison of the reparative and angiogenic properties of mesenchymal stem cells derived from the bone marrow of BALB/c and C57/BL6 mice in a model of limb ischemiaCharacterization of bone marrow mesenchymal stem cells. (a) Differentiation assay for bone marrow mesenchymal stem cells (MSCs): ischemic animals treated with MSCs obtained from C57/BL6 mice (MSC-Bl; I) and ischemic animals treated with MSCs obtained from BALB/c mice (MSC-Ba; IV). Adipogenic differentiation was evaluated by the formation of lipid vacuoles stained with Oil Red (II, V). The formation of mineralized matrix that stains with Alizarin Red provided evidence of osteogenic differentiation (III, VI). (b) Vascular endothelial growth factor (VEGF) gene expression analysis. Comparison of expression between MSC-Bl and MSC-Ba was performed by quantitative real-time PCR. *P <0.05. Bar: 50 μm.Back to article page