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Figure 1 | Stem Cell Research & Therapy

Figure 1

From: Generation and characterization of transgene-free human induced pluripotent stem cells and conversion to putative clinical-grade status

Figure 1

Representative colonies from the three hSTEMCCA-derived induced pluripotent stem cell lines. (A) Only the C8 pre-excised induced pluripotent stem cell (iPSC) line was found to have one integration into the PRPF39 gene and was therefore selected to undergo Adeno-Cre-PuroR selection for removal of the cassette. (B) Excision of hSTEMCCA from the pre-excised iPSC adult parental line (C8). RT-PCR of genomic DNA with primers against hSTEMCCA elements endo-Myc-s and A-WPRE, showing that one subclone (2.3 post-excised iPSCs) was free of the integrated provirus. Bars = 100 μM.

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Stem Cell Research & Therapy

ISSN: 1757-6512