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Figure 4 | Stem Cell Research & Therapy

Figure 4

From: Generation and characterization of transgene-free human induced pluripotent stem cells and conversion to putative clinical-grade status

Figure 4

Differentiation into therapeutically relevant cell lineages. (A) Post-excised induced pluripotent stem cells (iPSCs) were differentiated into oligodendrocyte progenitor cells expressing characteristic progenitor stage markers. (B) Upon terminal differentiation, the progenitor cells matured and displayed the mature antigen O1 and also stained positive for secreting myelin basic protein (MBP), a hallmark of mature oligodendrocytes. (C) Due to low efficiency of in vitro oligodendrocyte maturation, a co-culture system with dorsal root ganglion neurons was utilized and showed mature oligodendrocytes intimately associated with, and myelinating, neurite outgrowths. Additionally, human mitochondria were stained to display that rat oligodendrocytes were not contaminating the culture. (D) Hepatocytes were derived that stained positively for glycogen synthesis as indicated by the periodic acid–Schiff stain, and CK18, albumin, and alpha-fetoprotein. (E) Derived fibroblasts stained positive for COL3A1 upon differentiation and at levels comparable with those of control fibroblasts (left picture is iPSC-derived fibroblasts and right are control fibroblasts). (F) Cardiomyocytes showed expression of alpha-actinin and Troponin 1. DAPI, 4′,6-diamidino-2-phenylindole. Bars = 50 μM.

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