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Figure 5 | Stem Cell Research & Therapy

Figure 5

From: MicroRNA-21 promotes the ovarian teratocarcinoma PA1 cell line by sustaining cancer stem/progenitor populations in vitro

Figure 5

MiR-21 is required for PA1 CD133+ CSPC function. Purified CD133– and CD133+ populations from miR-lentivector, miR-21 KD, miR-cGFPvector and miR-21 OE were collected and the percentage of green fluorescence expression was detected by flow cytometry. The percentage of green fluorescence expression of infected cells was compared with that of non-infected cells. Flow cytometric signal intensities of non-infected cells were compared with those of infected cell populations (left-hand side). The percentages of infected cells with green fluorescence are presented on the right-hand side (A). The relative expression of miR-21 in CD133+ and CD133– cells was detected using quantitative real-time PCR. Expression of miR-21 in CD133– cells (basal level) was compared with that of miR-21 expression in CD133+ cells (B). Sphere formation assay images of the CD133+ and CD133– population colonies. The sphere colony images were taken under a phase contrast fluorescence microscope (100×). The images in the upper panel indicate vector-infected cells (left-hand side) and those on the right-hand side indicate anti-miR21-infected cells. The images of visible light and green fluorescence in the lower panel indicate vector-infected cells (left-hand side) and those on the right-hand side indicate miR21 overexpression (C). The right panel represents quantitation of sphere diameters. Data in (A) and (B) represent the mean of three individual sets of experiments. The variations were from the calculation of standard deviation. The scale bar represents 50 μm. CSPC, cancer stem/progenitor cell; KD: knockdown miR, microRNA; OE.

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