Cytosolic Ca2+regulates expression of cyclin B1. (A) Immunoblot analysis of cells that were transduced with the Ad-PV-NES-DsRed construct. The expression of cyclin B1 was decreased in the presence of the PV-NES-DsRed protein, but no changes were observed in the expression of the cell-cycle checkpoint proteins cyclins A, D1, D2, D3, E, or E2. GAPDH was used as a loading control. (B) Bar-graph summary showing that Ad-PV-NES-DsRed decreased the expression of cyclin B1 by 86% ± 7% (n = 3). *P < 0.05.