Cytosolic Ca2+regulates Erk1/2 phosphorylation. (A) Immunoblot analysis of cells that were transduced with the Ad-PV-NES-DsRed construct. Erk1/2 phosphorylation was decreased in the presence of the PV-NES-DsRed protein. Total Erk1/2 was used as a loading control. (B) Bar-graph summary showing that Ad-PV-NES-DsRed decreased Erk1/2 phosphorylation by 69% ± 5% (n = 3). *P < 0.05.