Figure 3

hES cell-derived neurons exhibit mature action potential responses. (A) Representative traces of action potentials fired in response to depolarization at 1, 2, 3, and 4 weeks after re-plating for terminal differentiation. Approximately 1 out of 7 and 1 out of 3 cells fired repetitive trains of action potentials at 3 and 4 weeks, respectively. (B) Quantification of the maximum action potential amplitude presented as mean ± SEM. A significant increase was observed from 1 to 2 weeks and again from 2 to 3 weeks. n = 19 (1w), 23 (2w), 26 (3w), and 47 (4w). *P <0.05, ***P <0.001.