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Figure 1 | Stem Cell Research & Therapy

Figure 1

From: Atoh7 promotes the differentiation of retinal stem cells derived from Müller cells into retinal ganglion cells by inhibiting Notch signaling

Figure 1

Purity of enriched Müller cells. Rat retinal Müller cells were enriched and passaged to obtain a highly purified cell population (A, B). Scale bar: 100 μm. Immunocytochemical and FACS analysis showed that more than 98% of purified cells were immunoreactive to Müller cell marker GS (C, D). Scale bar: 100 μm. The purity of enriched cells was evaluated by RT-PCR analysis to detect the expression of Müller cell specific transcripts (E) and other cell type specific transcripts (F). Lane M: DNA marker; lane 1: PN21 retina; lane 2: purified Müller cells. FACS, fluorescence-activated cell sorting.

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