Figure 1From: Atoh7 promotes the differentiation of retinal stem cells derived from Müller cells into retinal ganglion cells by inhibiting Notch signalingPurity of enriched Müller cells. Rat retinal Müller cells were enriched and passaged to obtain a highly purified cell population (A, B). Scale bar: 100 μm. Immunocytochemical and FACS analysis showed that more than 98% of purified cells were immunoreactive to Müller cell marker GS (C, D). Scale bar: 100 μm. The purity of enriched cells was evaluated by RT-PCR analysis to detect the expression of Müller cell specific transcripts (E) and other cell type specific transcripts (F). Lane M: DNA marker; lane 1: PN21 retina; lane 2: purified Müller cells. FACS, fluorescence-activated cell sorting.Back to article page