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Figure 2 | Stem Cell Research & Therapy

Figure 2

From: Atoh7 promotes the differentiation of retinal stem cells derived from Müller cells into retinal ganglion cells by inhibiting Notch signaling

Figure 2

Stem cell properties of enriched Müller cells. Enriched Müller cells exposed to the stem cell-conditioned medium formed neurospheres (A, B). The neurospheres were passaged to get new clonal neurospheres (C). Immunofluorescence staining showed that the stem cells within the cell spheres had positive expression of retinal stem cell-specific markers Nestin (92.94 ± 6.48%) and Pax6 (85.96 ± 6.04%) (D, F). Immunocytochemical analysis of Edu showed that newborn cell spheres had the capacity of effective proliferation (82.80 ± 6.65%) (E, F). Scale bar: 100 μm. RT-PCR and Western blot analysis to detect the expression of stem cell markers Nestin and Pax6 (G, H). Lane M: DNA marker; lane 1: Müller cells; lane 2: neurospheres.

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