Figure 4

The percentage of ganglion cells differentiated from retinal stem cells. A. The percentage of ganglion cells differentiated from retinal stem cells was 23.3 ± 4.45% (group a1), 50.6 ± 7.04% (Group a2) and 49.7 ± 7.36% (Group a3). B. The percentage of ganglion cells differentiated from retinal stem cells was 25.9 ± 3.35% (Group b1), 49.9 ± 5.38% (Group b2) and 49.2 ± 4.64% (Group b3). C. The percentage of ganglion cells differentiated from retinal stem cells was 58.2 ± 6.46% (Group c1) and 49.4 ± 5.78% (Group c2). D. Atoh7 and gamma-secretase inhibitor (GSI) were synergistic to promote the differentiation of retinal stem cells derived from Müller cells into retinal ganglion cells (F = 6.533, P = 0.005). E. RT-PCR showed that the mRNA expression of Brn-3b, Isl-1 and Notch1 was reduced in Brn-3b siRNA group, Isl-1 siRNA group and GSI group, respectively, at different time points (0 day, 1 day, 3 days, 7 days, 14 days). Atoh7 and GSI synergistically inhibited mRNA expression of Notch1.