Figure 5From: Atoh7 promotes the differentiation of retinal stem cells derived from Müller cells into retinal ganglion cells by inhibiting Notch signalingAtoh7 and GSI synergistically inhibit protein expression of Notch1 in retinal stem cells. The protein expression of Brn-3b, Isl-1 and Notch1 was reduced in Brn-3b siRNA group, Isl-1 siRNA group and GSI group, respectively, at different time points (0 day, 1 day, 3 days, 7 days, 14 days). Lane M: DNA marker; lane Con = retinal stem cells untreated; lane S1: retinal stem cells transfected with Brn-3b siRNA and PGC-FU-Atoh7-GFP; lane NC1: retinal stem cells transfected with scrambled siRNA and PGC-FU-Atoh7-GFP; lane S2: retinal stem cells transfected with Isl-1 siRNA and PGC-FU-Atoh7-GFP; lane NC2: retinal stem cells transfected with scramble siRNA and PGC-FU-Atoh7-GFP; lane S3: retinal stem cells treated with GSI and transfected with PGC-FU-Atoh7-GFP; lane NC3: retinal stem cells transfected with PGC-FU-Atoh7-GFP. β–actin was loading control.Back to article page