Skip to main content
Figure 5 | Stem Cell Research & Therapy

Figure 5

From: Atoh7 promotes the differentiation of retinal stem cells derived from Müller cells into retinal ganglion cells by inhibiting Notch signaling

Figure 5

Atoh7 and GSI synergistically inhibit protein expression of Notch1 in retinal stem cells. The protein expression of Brn-3b, Isl-1 and Notch1 was reduced in Brn-3b siRNA group, Isl-1 siRNA group and GSI group, respectively, at different time points (0 day, 1 day, 3 days, 7 days, 14 days). Lane M: DNA marker; lane Con = retinal stem cells untreated; lane S1: retinal stem cells transfected with Brn-3b siRNA and PGC-FU-Atoh7-GFP; lane NC1: retinal stem cells transfected with scrambled siRNA and PGC-FU-Atoh7-GFP; lane S2: retinal stem cells transfected with Isl-1 siRNA and PGC-FU-Atoh7-GFP; lane NC2: retinal stem cells transfected with scramble siRNA and PGC-FU-Atoh7-GFP; lane S3: retinal stem cells treated with GSI and transfected with PGC-FU-Atoh7-GFP; lane NC3: retinal stem cells transfected with PGC-FU-Atoh7-GFP. β–actin was loading control.

Back to article page