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Figure 1 | Stem Cell Research & Therapy

Figure 1

From: Mesenchymal stem cells and their chondrogenic differentiated and dedifferentiated progeny express chemokine receptor CCR9 and chemotactically migrate toward CCL25 or serum

Figure 1

MSC isolation, confirmation, and chondrogenic differentiation. (A) MSCs appeared as single cells in P0, and (B) showed uniform growth and fibroblast-like morphology in P3. (C) Flow-cytometric analysis revealed positive expression for typical MSC antigens like CD166, CD105, CD90, CD73, and CD44, but negative expression for hematopoietic lineage-specific antigens like CD45, CD34, and CD14 (three biologic replicates; n = 3), mean ± SEM. (D) On chondrogenic differentiation, they showed positive expression for Alcian blue staining compared with (E) control. (F) Similarly, they were positive for collagen type II expression compared with (G) unstimulated samples. (H) On the gene level, the chondrogenic differentiation was confirmed by significantly upregulated expression of COL2A1 and SOX9 genes compared with negative controls and undifferentiated MSCs, day 0 (n = 3). Student t test was performed for statistical analysis, and asterisks were assigned in the order P* < 0.05, P** < 0.01, and P*** < 0.001; mean ± SEM. Bar A, B, 500 μ m; D through G, 200 μ m.

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