Figure 2

Long-term engraftment and global migration of human central nervous system stem cells. (A) Saggital section of a NOD-SCID mouse brain transplanted as a neonate with human central nervous system stem cells (HuCNS-SC). Human cells were detected by immunoperoxidase staining with a human specific monoclonal antibody, STEM121 (brown), which demonstrates long-term engraftment and global migration within the brain. After transplantation into the lateral ventricles, human cells reside primarily in the neurogenic niche of the subventricular zone (SVZ). Migration radially from the SVZ is observed, including through the rostral migratory stream (RMS) to the olfactory bulb (OB). Local host signals define the predominant maturational outcomes of the cells. (B) Differentiation of HuCNS-SC to granular neurons in the olfactory bulb.