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Figure 6 | Stem Cell Research & Therapy

Figure 6

From: Clinical translation of human neural stem cells

Figure 6

Human central nervous system stem cells (HuCNS-SC) are cultured adherently to confluence on poly-ornithine coated (PLO) plates and the cells removed by water lysis and repeated cell culture buffer washes, leaving only their deposited extracellular matrix (ECM) behind. Subsequently, a human neural stem cell type derived by a different protocol than the one used for the HuCNS-SC was seeded on the ECM-coated plates under neuronal differentiation-inducing conditions. Coating with PLO only and double-coating with PLO and laminin served as negative and positive controls for a neurite outgrowth-promoting substrate, respectively. The extent of neurite outgrowth was quantified with high-content assay analysis software after 96 hours. Neurite length index: total length of detected neurites in micrometers per 100 nuclei. Data represents the results of two independent experiments in five replicate wells.

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